UAS regulatory sequences drive expression of the Hsap\GUK1 open reading frame, tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF was obtained from the human ORFeome v#N/A collection ( ORF_ID:4847 ) and corresponds to Ensembl transcript ENST00000312726.8). The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
