Principal Investigators Hugo Bellen and Susan Celniker, working with Shinya Yamamoto and Hillary Graves, are producing a new resource for expressing ~8,000 validated high-quality full-length human protein coding HA-tagged ORF cDNAs of genes conserved between human and Drosophila. Selected genes include those associated with known human diseases, those with fly homologs that can be easily manipulated with available tools, and those prioritized by other researchers (Drosophila biologists and human geneticists). The UAS-cDNA clones are being generated using either the pUASg-HA.attB or pGW-HA.attB vector (from Konrad Basler). Clones will be available from DGRC. In addition, the group will produce ~1,500 transgenic fly lines from a subset of these clones. The UAS-cDNAs are being inserted into specific loci (attP86Fb, VK33 or VK37) using the phiC31 integrase. Transgenic fly stocks will be available from the BDSC. The files associated with this personal communication provides details for these transgenic fly stocks. The human cDNA clones and corresponding fly strains will allow Drosophila and human researchers to test the functional replacement of Drosophila genes with human homologs and assess if human variants affect gene function.