FlyBase Allele Report: Dmel\Wdfy2[R323A.UAS.mCherry]

General Information

Symbol

Dmel\Wdfy2^{R323A.UAS.mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0337140

Feature type

allele

Associated gene

Dmel\Wdfy2

Associated Insertion(s)

Carried in Construct

P{UAS-mCherry.Wdfy2^R323A}

Key Links

Transgenic product class

missense_variant

(O'Farrell et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(O'Farrell et al., 2017)

Mutagen

in vitro construct

(O'Farrell et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-mCherry.Wdfy2^R323A}

Cytology

Description

UAS regulates expression of Wdfy2 containing a R323A substitution in the FYVE domain, tagged at the N-terminus with mCherry.

(O'Farrell et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Wdfy2

Tagged with

mCherry

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Phenotype Manifest In

wing, with Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Detailed Description

Statement

Reference

The expression of Wdfy2^{R323A.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ap-md544 leads to adult wings presenting an upward curvature.

(O'Farrell et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, Wdfy2^{R323A.UAS.mCherry} has visible phenotype, enhanceable by Lkb1^UASp.GFP, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, Wdfy2^{R323A.UAS.mCherry} has visible phenotype, suppressible by Lkb1^HMS01351, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Enhancer of

Statement

Reference

Wdfy2^{R323A.UAS.mCherry}, Scer\GAL4^ap-md544 is an enhancer of visible phenotype of Lkb1^UASp.GFP, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, Wdfy2^{R323A.UAS.mCherry} has wing phenotype, enhanceable by Lkb1^UASp.GFP, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, Wdfy2^{R323A.UAS.mCherry} has wing phenotype, suppressible by Lkb1^HMS01351, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Enhancer of

Statement

Reference

Wdfy2^{R323A.UAS.mCherry}, Scer\GAL4^ap-md544 is an enhancer of wing phenotype of Lkb1^UASp.GFP, Scer\GAL4^ap-md544

(O'Farrell et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The upward adult wing curvature resulting from the expression of Wdfy2^{R323A.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ap-md544 is suppressed by the co-expression of Lkb1^HMS01351.

The co-expression of Lkb1^{Scer\UAS.P\T.T:Avic\GFP} and Wdfy2^{R323A.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ap-md544 enhances the wing defects observed upon either single expression.

(O'Farrell et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Wdfy2^{R323A.Scer\UAS.T:Disc\RFP-mCherry}

Wdfy2^{R323A.UAS.mCherry}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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