FlyBase Allele Report: Dmel\whd[TOE.GS00536]

General Information

Symbol

Dmel\whd^TOE.GS00536

Species

D. melanogaster

Name

FlyBase ID

FBal0337690

Feature type

allele

Associated gene

Dmel\whd

Associated Insertion(s)

Carried in Construct

P{TOE.GS00536}

Key Links

Transgenic product class

sgRNA

(Transgenic RNAi Project members, 2017-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Transgenic RNAi Project members, 2017-)

Mutagen

in vitro construct

(Transgenic RNAi Project members, 2017-)

Progenitor genotype

Carried in construct

P{TOE.GS00536}

Cytology

Description

CRISPR construct designed to result in activation of a target gene when combined with a source of nuclease-dead Cas9 fused to a transcriptional activator domain. Constitutively expresses two sgRNAs, both of which target sequence upstream of the transcription start site of whd.

(Transgenic RNAi Project members, 2017-)

Allele components

Component

Use(s)

Encoded product / tool

sgRNA-GS00536.1

sgRNA-GS00536.2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | larval stage, with Scer\GAL4^dome-PG14

(Tiwari et al., 2020)

decreased occurrence of cell division | larval stage, with Scer\GAL4^dome-PG14

(Tiwari et al., 2020)

Phenotype Manifest In

primary lobe of embryonic/larval lymph gland | larval stage, with Scer\GAL4^dome-PG14

(Tiwari et al., 2020)

secondary lobe of embryonic/larval lymph gland | larval stage, with Scer\GAL4^dome-PG14

(Tiwari et al., 2020)

Detailed Description

Statement

Reference

Expression of whd^TOE.GS00536 under the control of Scer\GAL4^dome-PG14 (in combination with tub-Gal80[ts]) leads to an increase in the differentiation of hemocyte progenitors in both primary and secondary lobes of larval lymph gland. It also leads to a decrease in the occurrence of cell division in primary lobe of larval lymph gland.

(Tiwari et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^dome-PG14/whd^TOE.GS00536 is a suppressor of abnormal size | larval stage phenotype of Scer\GAL4^dome-PG14, bsk^DN.UAS

(Tiwari et al., 2020)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^dome-PG14/whd^TOE.GS00536 is a suppressor of cortical zone of lymph gland primary lobe | larval stage phenotype of Scer\GAL4^dome-PG14, bsk^DN.UAS

(Tiwari et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

68139

y¹ sc^* v¹ sev²¹; P{TOE.GS00536}attP40/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

whd^TOE.GS00536

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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