UAS regulatory sequences drive expression of ase cDNA (codon optimized for Drosophila, omitting endogenous 5' and 3' UTR) with replacement of the C-terminal with the en repressor domain. Tagged with Tag:MYC.
The T1 and T2 segments of stage 16 embryos expressing aseEnR.UAS.Tag:MYC under the control of Scer\GAL4elav-C155 show significant decreases in the numbers of dividing neuroblasts and dividing neuroblast daughters, as compared to controls.