Genomic fragment encompassing the entire unc-13 locus including putative promoter regions (derived from Pacman BAC clone CH321-60O10). The first 1000bp of the B-isoform specific N-terminal exon have been deleted, resulting in a frameshift that interrupts translation of the B isoform by producing a premature stop codon.
Genomic fragment encompassing the entire unc-13 locus including putative promoter regions (derived from Pacman BAC clone CH321-60O10). The first 1000bp of the B-isoform specific N-terminal exon have been deleted, resulting in a frameshift that interrupts translation of the unc-13-RB and unc-13-RE isoforms by producing a premature stop codon.
unc-13P84200 homozygotes bearing unc-13Bnull are viable and fertile.
The third instar larval neuromuscular junction of unc-13P84200 homozygotes bearing unc-13Bnull exhibit a significant decrease in active zone (identified by Bruchpilot puncta) density, but not size, as compared to controls; neurotransmission at these neuromuscular junctions exhibit a significant decrease in the amplitude, but not kinetics, of evoked excitatory currents, and a significant increase in decay time, but not in amplitude or frequency, of miniature excitatory currents, as compared to controls.
