FlyBase Allele Report: Dmel\Pc[G4DBD.GMR]

General Information

Symbol

Dmel\Pc^G4DBD.GMR

Species

D. melanogaster

Name

FlyBase ID

FBal0340259

Feature type

allele

Associated gene

Dmel\Pc

Associated Insertion(s)

Carried in Construct

P{GMR.GAL4-Pc}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pham et al., 2008)

Progenitor genotype

Pc^G4DBD.cMa.hs

(Pham et al., 2008)

Carried in construct

P{GMR.GAL4-Pc}

Cytology

Description

GMR regulatory sequences and a heat shock inducible Hsp70 promoter regulate expression of Heat-shock and GMR drive expression of the Scer\GAL4 DNA-binding domain (amino acids 4-147) fused to the transcriptional repressor domain of Pc (amino acids 3-390).

(Pham et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | heat sensitive, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

lethal - all die during embryonic stage | heat sensitive

(Pham et al., 2008)

some die during embryonic stage | heat sensitive

(Pham et al., 2008)

some die during pupal stage | heat sensitive, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

Phenotype Manifest In

adult optic lobe, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

eye, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

lamina, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

ommatidium, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

retina, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

rhabdomere, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

rhabdomere membrane, with tsr^Δ96, tsr^UAS.int

(Pham et al., 2008)

Detailed Description

Statement

Reference

Expression of Scer\GAL4^GMR.Pc in a wild-type background does not affect the eye.

Heat-shock overexpression of Scer\GAL4^GMR.Pc is lethal, but overexpression from the larval stage onward doesn't have a significant effect on development or viability.

(Pham et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Pc^G4DBD.GMR, tsr^Δ96, tsr^UAS.int has eye phenotype, enhanceable by ssh^26-1/ssh[+]

(Pham et al., 2008)

Pc^G4DBD.GMR, tsr^Δ96, tsr^UAS.int has eye phenotype, enhanceable by flr[+]/flr^BG2697R5

(Pham et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Pc^G4DBD.GMR/tsr^UAS.int rescues tsr^Δ96

(Pham et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pc^G4DBD.GMR

Scer\GAL4^GMR.Pc

Name Synonyms

Secondary FlyBase IDs

FBal0246828
FBal0340225

References (2)

Report Sections

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