FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\KulCR00477-TG4.1
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General Information
Symbol
Dmel\KulCR00477-TG4.1
Species
D. melanogaster
Name
FlyBase ID
FBal0340614
Feature type
allele
Associated gene
Dmel\Kul
Associated Insertion(s)
TI{CRIMIC.TG4.1}KulCR00477-TG4.1
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Gene Disruption Project members, 2018-)
gene targeting by homologous recombination
(Gene Disruption Project members, 2018-)
Progenitor genotype
Associated Insertion(s)
TI{CRIMIC.TG4.1}KulCR00477-TG4.1
Cytology
Description

A TI{CRIMIC.TG4.1} DNA cassette has been inserted into Kul, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TCAGAAGATGAAGTCAGCACAGG. The homology arms of the donor vector overlapped by 9 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.

(Levis, 2019.6.28)
Allele components
Component
Use(s)
Inserted element
TI{CRIMIC.TG4.1}
Encoded product / tool
GAL4
Also carries
attP
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      KulCR00477-TG4.1 embryos do not show blood-brain barrier or trachea barrier as, like in controls, dye injected into the body cavity does not diffuses into the ventral nerve cord of into the tracheal tubes; the trachea also presents a normal morphology and normal distribution of the septate junction protein, NrxIV.

      (Petri et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      KulCR00477-TG4.1
      Name Synonyms
      Secondary FlyBase IDs
        References (3)