FB2026_03 , released September 17, 2026
Allele: Dmel\ClC-bEGFP
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General Information
Symbol
Dmel\ClC-bEGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0341720
Feature type
allele
Associated gene
Dmel\ClC-b
Associated Insertion(s)
TI{TI}ClC-bEGFP
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Wong et al., 2017)
gene targeting by homologous recombination
(Wong et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}ClC-bEGFP
Cytology
Description

In frame EGFP knock-in at the N-terminus (after the start codon) of ClC-b.

(Wong et al., 2017)
Allele components
Component
Use(s)
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      larval central nervous system
      (Wong et al., 2017)
      embryonic/larval Malpighian tubule
      (Wong et al., 2017)
      embryonic/larval gut
      (Wong et al., 2017)
      larval somatic muscle cell
      (Wong et al., 2017)
      larval pericardial cell
      (Wong et al., 2017)
      embryonic/larval lymph gland
      (Wong et al., 2017)
      embryonic/larval plasmatocyte
      (Wong et al., 2017)
      embryonic/larval fat body
      (Wong et al., 2017)
      Additional Information
      Statement
      Reference

      Tagged ClC-b was expressed in many tissues including the nervous system, Malpighian tubules, gut, skeletal muscles, and pericardial cells. Particularly strong enrichment was observed in the lymph glands, macrophages (plasmatocytes), and fat bodies.

      (Wong et al., 2017)
       
      Marker for
      Reflects expression of
      ClC-b
      (Wong et al., 2017)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      ClC-bEGFP individuals do not exhibit significant changes in bacterial load after injection with E.coli, as compared to equally infected controls.

      (Wong et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ClC-bEGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)