The PCR check of the insertion gave no band for the right flank and the expected sized product for the left flank.
A TI{CRIMIC.GT36} DNA cassette has been inserted into sws, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.GT36} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: GCCATAGGACTGAGATGCTGCGG.