FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sema1aMI00031-AE
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General Information
Symbol
Dmel\Sema1aMI00031-AE
Species
D. melanogaster
Name
FlyBase ID
FBal0343228
Feature type
allele
Associated gene
Dmel\Sema1a
Associated Insertion(s)
Mi{Sema1a.AE}Sema1aMI00031-AE
Carried in Construct
Also Known As
sema-1a artificial exon
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
site specific recombination
(Pecot et al., 2013)
Progenitor genotype
Mi{MIC}Sema1aMI00031
(Pecot et al., 2013)
Associated Insertion(s)
Mi{Sema1a.AE}Sema1aMI00031-AE
Cytology
Description

The attP cassette of the Mi{MIC} element has been replaced with an artificial exon that contains: 1) splice acceptor sequence, 2) Sema1a coding sequence, 3) FRT-stop-FRT, 4) sequences encoding 3xTag:V5, 5) 2A peptide, 6) lexA::p65, and 7) 3' UTR of Sema1a. In the absence of FLP, a transcription termination sequence prevents expression of tagged Sema1a, but when FLP is expressed the stop sequence is excised, allowing a Tag:V5 epitope to be added to the endogenous Sema1a protein, along with co-transcription of lexA::p65 and Sema1a.

(Pecot et al., 2013)
Allele components
Component
Use(s)
Encoded product / tool
lexA::p65
Tagged with
Tag:V5
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Sema1aMI00031-AE
      Name Synonyms
      Secondary FlyBase IDs
        References (3)