FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\VAChTY49N
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General Information
Symbol
Dmel\VAChTY49N
Species
D. melanogaster
Name
FlyBase ID
FBal0343517
Feature type
allele
Associated gene
Dmel\VAChT
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Vernon et al., 2018)
gene targeting by homologous recombination
(Vernon et al., 2018)
Progenitor genotype
Cytology
Description

Amino acid replacement: Y49N.

(Vernon et al., 2018)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:18,712,954..18,712,954 [+]
Nucleotide change:

T18712954A

Amino acid change:

Y49N | VAChT-PA

Reported amino acid change:

Y49N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Vernon et al., 2018)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      VAChTY49N heterozygotes and homozygotes exhibit a significant reduction in 5-Cl-CASPP toxicity (reduced mortality) compared to controls. They also exhibit a significant increase in the mini frequency of third instar larval aCC/RP2 motor neurons compared to controls.

      VAChTY49N homozygous third instar larvae exhibit significantly reduced endogenous burst frequency of aCC/RP2 motoneurons compared to controls.

      The dorsal longitudinal flight muscle of VAChTY49N adults is less able to follow a train of 10 stimuli during high frequency stimulation (100Hz) compared to controls.

      At an optogenetic stimulation frequency of 1Hz, homozygous VAChTY49N third instar larval aCC/RP2 motoneurons exhibit a significantly reduced time until first synaptic failure compared to controls. The ratio change in current density between the 1st and 2nd EPSC is also significantly reduced. The minimum time interval required between successive stimulations to generate a second full size EPSC at 100% in VAChTY49N homozygotes is approximately doubled compared to controls.

      VAChTY49N homozygous larvae exhibit defects in larval crawling with significantly reduced total distance travelled compared to controls.

      (Vernon et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      VAChTY49N
      (Goodchild et al., 2024, Vernon et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)