PAPLA1¹ mutants are semi-lethal and show a delay to pupariation, but do not show differences in body size (assessed by pupal volume and adult wing size); adults show significant decreases in food intake an din spontaneous activity, as compared to controls. Mutant females have smaller ovaries, which show increased egg chamber degeneration at mid-oogenesis and occasional persistence of nurse cell nuclei after stage 13 of oogenesis, but no other obvious morphology defects, as compared to controls; their mating to control males leads to significantly fewer eggs being laid, which hatch at a slightly but significantly lower rate, as compared to controls. Mutant males show smaller reproductive organs (i.e. testes and accessory glands) and their mating to control females leads to decreased egg laying and egg hatch rate, as compared to control males.