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General Information
Symbol
Dmel\mei-9dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0344492
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulates expression of a RNAi construct targeting mei-9.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In individuals expressing mei-9dsRNA.UAS under the control of Scer\GAL4Act88F.1.3, the adult longitudinal thoracic muscles exhibit increased DNA damage (phospho-H2AvD staining) and premature aging (progressive loss of proteostasis) but do not show signs of profound tissue damage (as in muscle structure or function); individuals do not show defects in feeding behavior or in climbing. Their adult midguts show increased mitosis (i.e. a significant increase in 30-days old adults, but not in 10- or 15-days old adults) but posterior midgut clones progressively lose cells (significant decrease in 30-days old adults, but not in 10-days old adults), as compared to controls.

Expression under the control of Scer\GAL4A58 also leads to DNA damage repair defects, as UV irradiation of larvae leads to visible abdomen cuticle defects that are not present in irradiated controls. Expression under the control of Scer\GAL4NP0001 leads to the adult midgut accumulating DNA damage (phospho-H2AvD staining) and exhibiting a progressive increase in mitosis, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference

Scer\GAL4A58, mei-9dsRNA.UAS has adult abdomen | conditional phenotype, non-suppressible by Ercc1KK101553, Scer\GAL4A58

Additional Comments
Genetic Interactions
Statement
Reference

In adults expressing mei-9dsRNA.UAS under the control of Scer\GAL4Act88F.1.3, longitudinal thoracic muscles exhibit DNA damage (phospho-H2AvD staining) and premature aging (progressive loss of proteostasis); both of these are robustly enhanced by the co-expression of Ercc1KK101553, which is highlighted by a premature loss of proteostasis and disruption of myofibril muscle structure during aging. 30-days adults expressing mei-9dsRNA.UAS also present increased mitosis in the midgut, which is also suppressed by the co-expression of Ercc1KK101553.

The UV-induced cuticle defects observed in the abdomen of adults expressing mei-9dsRNA.UAS under the control of Scer\GAL4A58 are not suppressed by the co-expression of Ercc1KK101553.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Obtained from J. Sekelsky.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
mei-9dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (1)