FB2026_02 , released June 18, 2026
Allele: Dmel\CG15615CR01055-TG4.0-X
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General Information
Symbol
Dmel\CG15615CR01055-TG4.0-X
Species
D. melanogaster
Name
FlyBase ID
FBal0345678
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
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Allele class
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Allele class
Progenitor genotype
Cytology
Description

A TI{CRIMIC.TG4.0} DNA cassette has been inserted into CG15615 using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The cassette is not predicted to trap CG15615 or express GAL4 because the CRISPR sgRNA was mistakenly targeted to a coding exon of the gene; no GAL4 expression was detected in the larval or adult nervous system. The sgRNA sequence used to target the gene was: TCTGCTCGGTGTGCTCTGTTTGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.0} cassette is predicted to be accompanied by a deletion of 868bp of genomic sequence flanking the insertion site. The PCR check of the insertion gave multiple bands for the left flank and the expected sized product for the right flank.

Mutations Mapped to the Genome
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Variant Molecular Consequences
Associated Sequence Data
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Protein sequence
 
Expression Data
Reporter Expression
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Marker for
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Reporter construct used in assay
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Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
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Xenogenetic Interactions
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Complementation and Rescue Data
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Stocks (1)
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External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
CG15615CR01055-TG4.0-X
Name Synonyms
Secondary FlyBase IDs
    References (1)