A TI{CRIMIC.TG4.0} DNA cassette has been inserted into Cdep, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.0} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: ATGTAAGTCCTCCTGGTATAGGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.0} cassette is predicted to be accompanied by a deletion of 4bp of genomic sequence flanking the insertion site.
CdepCR01137-TG4.0 is a suppressor | partially of abnormal neurophysiology | adult stage phenotype of Hsap\MAPTP301L.QUAS.0N4R, Ncra\QFQF2w.nSyb
CdepCR01137-TG4.0 is a suppressor | partially of retina | adult stage phenotype of Hsap\MAPTP301L.QUAS.0N4R, Ncra\QFQF2w.nSyb