FlyBase Allele Report: Dmel\ens[UAS.EGFP]

General Information

Symbol

Dmel\ens^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0346147

Feature type

allele

Associated gene

Dmel\ens

Associated Insertion(s)

Carried in Construct

P{UAS-ens.EGFP}

Key Links

Transgenic product class

cDNA

(Rosen et al., 2019)

wild_type

(Rosen et al., 2019)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Rosen et al., 2019)

wild_type

(Rosen et al., 2019)

Mutagen

in vitro construct

(Rosen et al., 2019)

Progenitor genotype

Carried in construct

P{UAS-ens.EGFP}

Cytology

Description

UASt regulatory sequences drive expression of full-length ens (sequence derived from the LD09626 cDNA clone). The coding sequence is tagged at the C-terminal end with EGFP.

(Rosen et al., 2019)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ens

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic somatic muscle cell | embryonic stage 16, with Scer\GAL4^Mef2.PR

(Rosen et al., 2019)

nucleus | embryonic stage 16, with Scer\GAL4^Mef2.PR

(Rosen et al., 2019)

Detailed Description

Statement

Reference

Expressing ens^UAS.EGFP under the control of Scer\GAL4^Mef2.PR results in stage 16 embryo myotubes showing abnormal nuclear positioning, namely nuclei spreading and traversing a shorter distance toward the muscle poles and nuclei often present as individuals rather than as part of clusters, as compared to controls.

(Rosen et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, ens^UAS.EGFP has nucleus | embryonic stage 16 phenotype, non-suppressible by Nin^UAS.mEosFP, Scer\GAL4^Mef2.PR

(Rosen et al., 2019)