FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sema1bKO
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General Information
Symbol
Dmel\Sema1bKO
Species
D. melanogaster
Name
FlyBase ID
FBal0346268
Feature type
allele
Associated gene
Dmel\Sema1b
Associated Insertion(s)
TI{TI}Sema1bKO
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Wittes and Schüpbach, 2019)
gene targeting by homologous recombination
(Wittes and Schüpbach, 2019)
Progenitor genotype
Associated Insertion(s)
TI{TI}Sema1bKO
Cytology
Description

The Disc\RFPDsRed(T1).3xP3.cHa marker that is inserted in TI{TI}Sema1bKO is flanked by loxP sites, and a single attP site is also present upstream of this loxP cassette.

(Wittes and Schüpbach, 2020.2.4)

A large part of the Sema1b coding region has been deleted and replaced by a sequence encoding a Disc\RFPDsRed(T1).3xP3.cHa marker.

(Wittes and Schüpbach, 2019)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2R:17,675,181..17,676,438 [-]
Comment:

A 1258bp deletion of part of the Sema1b coding region. The deleted sequences have been replaced by a sequence encoding a Disc\RFPDsRed(T1).3xP3.cHa marker.

(Wittes and Schüpbach, 2020.2.4)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Statement
      Reference

      Sema1bKO is a non-enhancer of abnormal neuroanatomy | P-stage phenotype of Scer\GAL4nSyb.PS, Sema1aHMS01307

      (Bustillo et al., 2024)
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      Sema1bKO is a non-enhancer of medulla layer | P-stage phenotype of Scer\GAL4nSyb.PS, Sema1aHMS01307

      (Bustillo et al., 2024)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Sema1bKO
      (Bustillo et al., 2024, Wittes and Schüpbach, 2020.2.4)
      Sema1bKO
      (Wittes and Schüpbach, 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)