Ndg^Δ1.4 adults exhibit incomplete wing inflation at low penetrance.

Ndg^Δ1.4 larvae show locomotor defects. First instar larvae move within a smaller area and display a variety of sudden motion defects in crawling pattern, such as head shaking, and spontaneous rolling and bending; when placed on agar plates supplemented with yeast paste as food source, Ndg^Δ1.4, Ndg^Δ1.4/Ndg^Δ0.4 and Ndg^Δ1.4/Df(2R)BSC281 first instar larvae are more frequently observed outside the food than controls. Ndg^Δ1.4 third instar larvae show locomotor defects compared to controls: lower speed and stride frequency, significantly lower climbing distance, and the orientation of pupal cases is significantly shifted to the horizontal axis, as compared to controls. Ndg^Δ1.4 third instar larvae also show severely decreased body length retraction in response to vibrational stimuli, as compared to controls.

In Ndg^Δ1.4 third instar larvae, the lateral pentascolopidial chordotonal organ frequently loses the alignment of sensory cilia, and the cell bodies of the dorsal dendritic arborization sensory neurons (ddA neurons) fail to align, resulting in significantly increased dda cluster area, a compared to controls. There is also severely reduced targeting of alary muscles by the dorsal projection of the transverse nerve, as compared to controls.

The third instar larval neuromuscular junction of Ndg^Δ1.4 individuals are altered in shape, display a slight but significant increase in size, display a significant size difference between corresponding junctions on both sides of one segment, and display significant increases in the number of branches, branch length and number of boutons, as compared to controls.

Although Ndg^Δ1.4/Df(2R)BSC281 embryos do not show obvious defects in the formation/overall assembly of basement membranes, larvae show basement membranes defects, are they are leaky (wing discs exhibit decreased resistance to osmotic stress) and show ultrastructure defects (small holes in the close vicinity of longitudinal visceral muscles on the midgut surface). Basement membranes in Ndg^Δ1.4 larvae are also leaky (somatic muscles are permeable to dextran and wing discs show decreased resistance to osmotic stress), and also show ultrastructure defects (small holes where tracheal branches attach to somatic muscles, in the close vicinity of longitudinal visceral muscles on the midgut surface, and in the fat body). Ultrastructure defects in the basement membrane (small holes in the visceral muscles) are also observed in Ndg^Δ1.4 heterozygotes but never in wild-type controls.