FlyBase Gene Report: Dmel\Ndg

General Information

Symbol

Dmel\Ndg

Species

D. melanogaster

Name

Nidogen

Annotation Symbol

CG12908

Feature Type

protein_coding_gene

FlyBase ID

FBgn0026403

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

Cell adhesion glycoprotein which is widely distributed in basement membranes (PubMed:30260959, PubMed:30567930). Involved in cell-extracellular matrix (ECM) interactions probably by connecting the laminin and collagen IV networks (PubMed:30260959, PubMed:30567930). Required for permeability and mechanical stability of basement membranes, and ECM dependent neural plasticity (PubMed:30567930). Not involved in assembly of the embryonic basement membrane (PubMed:30567930). (UniProt, A1Z877)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

Nidogen/entactin

Key Links

Genomic Location

Cytogenetic map

47A1-47A1

Sequence location

2R:10,313,891..10,322,104 [+]

Recombination map

2-62

RefSeq locus

NT_033778 REGION:10313891..10322104

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables extracellular matrix structural constituent

inferred from mutant phenotype

(Dai et al., 2018)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables calcium ion binding

inferred from electronic annotation with InterPro:IPR001881, InterPro:IPR018097

(InterPro Project Members, 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in basement membrane assembly involved in embryonic body morphogenesis

inferred from mutant phenotype

(Wolfstetter et al., 2019)

involved_in basement membrane organization

inferred from mutant phenotype

(Dai et al., 2018)

involved_in regulation of basement membrane organization

inferred from mutant phenotype

(Wolfstetter et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in cell-matrix adhesion

inferred from electronic annotation with InterPro:IPR003886

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in basement membrane

inferred from direct assay

(Dai et al., 2018)

inferred from direct assay

(Wolfstetter et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in basement membrane

traceable author statement

(Hynes and Zhao, 2000)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001346488

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

(UniProt, A1Z877)

Summary (Interactive Fly)

basement membrane component - required for proper assembly and maintenance of certain basement membranes of the adipose tissue and flight muscles - binds to and couples Collagen IV and Laminin networks

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ndg for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z877)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ndg-RA

FBtr0088349

NM_136731

4612

1350

Ndg-RC

FBtr0339459

NM_206074

4816

1350

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ndg-PA

FBpp0087438

149.1

1350

4.20

FBpp0308545

149.1

1350

4.20

A1Z877

NP_995796

AAS64880

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1350 aa isoforms: Ndg-PA, Ndg-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Domain

The NIDO domain (also known as globular region 1 or G1) contributes to the localization to the basal membrane probably by binding to vkg.

The EGF-like 1 and nidogen G2 beta-barrel domain (also known as globular region 2 or G2) contribute to the localization to the basal membrane probably by binding to vkg.

The EGF-like 2-8 domains (also known as the ROD domain) is necessary but not sufficient for localization to the basal membrane.

The LDL-receptor class B 1-4 domains (also known as globular region 3 or G3) is necessary for cell-extracellular matrix (ECM) interactions. Also, contributes to the localization to the basal membrane probably by binding to laminins.

(UniProt, A1Z877)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ndg using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ndg_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.80

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

muscle founder cell

(Philippakis et al., 2006)

circular trunk visceral muscle primordium

(Zhu et al., 2012)

embryonic stage 11 -- 12

cardiogenic mesoderm

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

embryonic head | restricted

(Wolfstetter et al., 2019)

gnathal segment | restricted

(Wolfstetter et al., 2019)

mesoderm | dorsal

(Wolfstetter et al., 2019)

embryonic myoblast

(Wolfstetter et al., 2019)

dorsal median cell

(Wolfstetter et al., 2019)

embryonic stage 11 -- 14

amnioserosa

(Wolfstetter et al., 2019)

embryonic stage 12

cardiogenic mesoderm

(Zhu et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

embryonic/larval esophageal visceral muscle

(Fisher et al., 2012)

embryonic stage 14

chordotonal organ

(Wolfstetter et al., 2019)

esophageal visceral muscle primordium

(Wolfstetter et al., 2019)

posterior midgut primordium | posterior | restricted

(Wolfstetter et al., 2019)

large intestine primordium | anterior | restricted

(Wolfstetter et al., 2019)

embryonic stage 16

visceral trunk mesoderm of embryonic/larval midgut

(Zhu et al., 2012)

larval somatic muscle cell

(Wolfstetter et al., 2019)

larval visceral muscle cell

(Wolfstetter et al., 2019)

scolopidial dendritic cap cell

(Wolfstetter et al., 2019)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 17

organism

(Wolfstetter et al., 2019)

Additional Descriptive Data

Northern blots show high levels of Ndg after gastrulation and increasing in older embryos. Ndg expression is observed by fluorescence in situ hybridization at embryonic stage 12 in single cells in the head, especially in the gnathal segments as well as in segmentally located patches of cells in the dorsal mesoderm. The midline-associated, mesodermal dorsal median cells (DMC) as well as four surrounding somatic myoblasts show strong Ndg expression. Weak expression is detected in the amnioserosa. After germband retraction, expression is visible in the forming dorsal and ventral muscles, the amnioserosa, and the segmentally located chordotonal organs. An internal view shows decreased expression in the DMCs and shows expression in the esophageal visceral muscle primordium and the joint region between the hindgut and the midgut. At embryonic stage 16, strong mRNA expression is seen in the somatic and visceral muscles and in the cap cells of the chordotonal organs. No expression is seen in hemocytes or fat body cells.

(Wolfstetter et al., 2019)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

presumptive cercus | surrounding

(Wolfstetter et al., 2019)

dorsal median cell | surrounding

(Wolfstetter et al., 2019)

embryonic myoblast | surrounding

(Wolfstetter et al., 2019)

embryonic stage 13

larval visceral muscle cell

• basement membrane

(Wolfstetter and Holz, 2012)

embryonic stage 14

embryonic brain

• basement membrane

(Wolfstetter et al., 2019)

larval ventral nerve cord

• basement membrane

(Wolfstetter et al., 2019)

larval tracheal system

• basement membrane

(Wolfstetter et al., 2019)

embryonic somatic muscle cell

• basement membrane

(Wolfstetter et al., 2019)

presumptive embryonic/larval digestive system

• basement membrane

(Wolfstetter et al., 2019)

embryonic stage 16

embryonic/larval midgut

• extracellular matrix

(Wolfstetter and Holz, 2012)

organism

• basement membrane

(Wolfstetter et al., 2019)

chordotonal organ

• basement membrane

(Wolfstetter et al., 2019)

larval ventral nerve cord

• basement membrane

(Wolfstetter et al., 2019)

larval brain

• basement membrane

(Wolfstetter et al., 2019)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Ndg protein, embedding dorsal median cells and somatic myoblasts, forms a thin sheet between ecto- and mesoderm, and around the prospective anal plate at embryonic stage 11/12. After germband retraction, Ndg protein is observed in the forming basement membranes around the developing brain and ventral nerve cord, in the differentiating tracheal system, in the digestive tract and in the forming somatic muscles. At embryonic stage 16, Ndg protein localizes to all embryonic basement membranes. Basement membranes surrounding the chordotonal organs, the ventral nerve cord, and the brain are highly Ndg protein-positive even though these tissues do not express Ndg mRNA.

(Wolfstetter et al., 2019)

At stage 13, Ndg staining is visible in distinct arrays of extracellular matrix at the edges of visceral muscles. By stage 16, Ndg is localized on the extracellular matrix next to the midgut.

(Wolfstetter and Holz, 2012)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in basement membrane

inferred from direct assay

(Dai et al., 2018)

inferred from direct assay

(Wolfstetter et al., 2019)

Expression Deduced from Reporters

Reporter: P{Ndg-GFP.WT}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

muscle founder cell

(Zhu et al., 2012)

embryonic stage 15 -- 17

embryonic/larval midgut muscle cell

(Zhu et al., 2012)

Reporter: P{Ndg-lacZ.P}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

muscle founder cell

(Zhu et al., 2012)

embryonic stage 15 -- 17

embryonic/larval midgut muscle cell

(Zhu et al., 2012)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ndg in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ndg

View exonic expression by tissue for Dmel\Ndg

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0026403

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36089/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0026403

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0026403

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0026403

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12908

Flygut - An atlas of the Drosophila adult midgut

FBgn0026403

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Ndg

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 22 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ndg

Transgenic constructs containing regulatory region of Ndg

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene partially disrupted in

(Dai et al., 2018)

(Dai et al., 2018)

(Dai et al., 2018)

(Dai et al., 2018)

(Dai et al., 2018)

(Dai et al., 2018)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal, with Scer\GAL4^tin.CΔ4

Ndg^GD5300

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ndg^GD5300

viable, with Scer\GAL4^Act.PU

Ndg^HMJ24142

viable, with Scer\GAL4^Mef2.PR

Ndg^GD5300

viable, with Scer\GAL4^pnr-MD237

Ndg^GD5300

Sterility

Allele

fertile

semi-fertile | female | progressive

Other Phenotypes

Allele

abnormal cold stress response | adult stage

abnormal cold stress response | adult stage (with Df(2R)BSC281)

abnormal flight, with Scer\GAL4^Mef2.PR

Ndg^GD5300

abnormal locomotor behavior | first instar larval stage

Ndg^Δ1.4

abnormal locomotor behavior | first instar larval stage (with Df(2R)BSC281)

Ndg^Δ1.4

abnormal locomotor behavior | first instar larval stage (with Ndg^Δ0.4)

Ndg^Δ1.4

abnormal locomotor behavior | first instar larval stage (with Ndg^Δ1.4)

Ndg^Δ0.4

abnormal locomotor behavior | third instar larval stage

abnormal neuroanatomy | third instar larval stage

Ndg^Δ1.4

abnormal sensory perception | third instar larval stage

abnormal size | larval stage

abnormal size | third instar larval stage

Ndg^Δ1.4

decreased body size | pupal stage

decreased fecundity | female

visible | adult stage

Ndg^Δ1.4

Phenotype manifest in

Allele

abdominal lateral pentascolopidial chordotonal organ lch5 | third instar larval stage

Ndg^Δ1.4

basement membrane | adult stage

Ndg¹

basement membrane | larval stage

basement membrane | larval stage, with Scer\GAL4^Cg.PA

Ndg^HMJ24142

basement membrane | larval stage (with Df(2R)BSC281)

basement membrane | larval stage (with Ndg¹)

basement membrane | larval stage (with Ndg²)

Ndg¹

basement membrane | larval stage (with Ndg^ΔRod-G3.1)

Ndg¹

dendritic arborizing neuron | third instar larval stage

Ndg^Δ1.4

embryonic/larval fat body | larval stage

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PA

Ndg^HMJ24142

embryonic/larval fat body | larval stage (with Df(2R)BSC281)

Ndg¹

embryonic/larval fat body | larval stage (with Ndg¹)

embryonic/larval fat body | larval stage (with Ndg²)

Ndg¹

embryonic/larval fat body | larval stage (with Ndg^ΔRod-G3.1)

Ndg¹

embryonic/larval midgut longitudinal visceral muscle cell | larval stage

embryonic/larval neuromuscular junction | third instar larval stage

Ndg^Δ1.4

indirect flight muscle cell | adult stage

Ndg¹

larval abdominal lch5 neuron | third instar larval stage

Ndg^Δ1.4

larval somatic muscle cell | larval stage (with Df(2R)BSC281)

Ndg^Δ1.4

larval transverse nerve | third instar larval stage

Ndg^Δ1.4

lipid droplet | larval stage

somatic muscle cell | larval stage

Ndg^Δ1.4

wing

Ndg^Δ1.4

wing disc

wing disc (with Df(2R)BSC281)

Ndg^Δ1.4

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (29)

Hsap\NID1

10 of 14

Yes

Hsap\NID2

9 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\NELL2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (24)

Rnor\Nid2

9 of 14

Yes

Rnor\Nid1

4 of 14

Yes

Rnor\Egf

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\LOC100911408

1 of 14

Yes

Rnor\LOC100911848

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (22)

Mmus\Nid1

9 of 14

Yes

Mmus\Nid2

9 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Scube2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (23)

Xtro\nid1

9 of 13

Yes

Xtro\rhobtb1

8 of 13

Yes

Xtro\nid2

5 of 13

Yes

Xtro\egf

2 of 13

Yes

Xtro\LOC100485083

2 of 13

Xtro\LOC101731739

2 of 13

Xtro\dlk1

1 of 13

Yes

Xtro\emx2

1 of 13

Xtro\glp2r

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (39)

Drer\nid1a

9 of 14

Yes

Drer\nid1b

9 of 14

Yes

Drer\nid2a

9 of 14

Yes

Drer\nid2b

4 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC100333187

1 of 14

Yes

Drer\LOC100536757

1 of 14

Yes

Drer\LOC101882968

1 of 14

Yes

Drer\LOC103910027

1 of 14

Yes

Drer\LOC103910028

1 of 14

Yes

Drer\LOC103911950

1 of 14

Drer\LOC108179152

1 of 14

Yes

Drer\LOC108191777

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\si:ch73-181m17.1

1 of 14

Drer\si:ch73-329n5.6

1 of 14

Yes

Drer\si:ch211-39f2.3

1 of 14

Drer\si:ch211-182p11.1

1 of 14

Yes

Drer\si:ch211-226h7.3

1 of 14

Yes

Drer\si:dkey-9l20.3

1 of 14

Yes

Drer\si:dkey-258f14.3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\zgc:153932

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\nid-1

11 of 14

Yes

Cele\B0393.5

2 of 14

Yes

Cele\dex-1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (13)

Agam\AgaP_AGAP008193

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (2)

Atha\WAK2

1 of 13

Yes

Atha\WAK5

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ndg. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (13)

arr

2 of 13

cue

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

mgl

2 of 13

crb

1 of 13

dpy

1 of 13

Megf8

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

10 of 14

9 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ndg - LanA

anti tag coimmunoprecipitation, western blot

(Rotstein et al., 2018)

Ndg - LanB1

anti tag coimmunoprecipitation, western blot

(Rotstein et al., 2018)

Ndg - vkg

anti tag coimmunoprecipitation, western blot

(Rotstein et al., 2018)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61908

DroID - A comprehensive database of gene and protein interactions.

FBgn0026403

MIST (protein-protein) - An integrated Molecular Interaction Database

36089

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-62

Cytogenetic map

47A1-47A1

Sequence location

2R:10,313,891..10,322,104 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

47A1-47A1

Limits computationally determined from genome sequence between P{lacW}Hr46^k10308 and P{PZ}lola⁰⁰³⁴⁹&P{EP}lola^EP952

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

47A1-47A2

(determined by in situ hybridisation)

(Baumgartner, 1999.3.10)

Experimentally Determined Recombination Data

Location

2-62

(FlyBase, 2016)

2-60.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29288

w¹¹¹⁸; Mi{ET1}Ndg^MB12298

61036

y¹ w^*; Mi{MIC}Ndg^MI15397

62902

y¹ v¹; P{TRiP.HMJ24142}attP40/CyO

66766

y¹ w^*; Mi{PT-GFSTF.0}Ndg^{MI15397-GFSTF.0}

76768

y¹ w^*; Mi{Trojan-GAL4.0}Ndg^{MI15397-TG4.0}/SM6a

NIG-Fly

12908R-2

P{NIG.12908R}2

12908R-3

P{NIG.12908R}3

HMJ24142

y¹ v¹; P{TRiP.HMJ24142}attP40/CyO

VDRC

v109625

P{KK101509}VIE-260B

v13280

w¹¹¹⁸; P{GD5300}v13280/TM3

v318629

PBac{fTRG00638.sfGFP-TVPTBF}VK00033

v342691

P{hsFLP}1, y1 w1118; P{HD_CFD01106}attP40/CyO-GFP

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (55)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0026403

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0026403

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Dai et al., 2018)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: Ndg CG12908

(Artero et al., 2003)

Source for merge of: CG12908 BEST:CK00552

(FlyBase, 1996-)

Additional comments

Source for merge of CG12908 BEST:CK00552 was sequence comparison ( date:030820 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

BEST:CK00552

CG12908

(Çiçek et al., 2016, Hosono et al., 2015, Cammarato et al., 2011, Bunt et al., 2010, Hurlbut et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Zinzen et al., 2009, Strub et al., 2008, Fakhouri et al., 2006, Zimmermann et al., 2006, Artero et al., 2003, Solano et al., 2003, Jasper et al., 2001, Mount, 2001.8.14)

CT32053

(Hynes and Zhao, 2000)

Ndg

(Lee et al., 2025, Santillán et al., 2025, Stricker et al., 2025, Töpfer and Holz, 2025, Li et al., 2024, Pignataro et al., 2024, Walther et al., 2024, Liu et al., 2022, Marcogliese et al., 2022, Ferguson et al., 2021, Garaulet et al., 2021, Itoh and Nishihara, 2021, McDonough-Goldstein et al., 2021, Sun et al., 2021, Aboukilila et al., 2020, Parra and Johnston, 2020, Pastor-Pareja, 2020, Töpfer and Holz, 2020, Zheng et al., 2020, Pastor J., 2019.2.13, Ramos-Lewis and Page-McCaw, 2019, Wolfstetter et al., 2019, Dai et al., 2018, Ramos-Lewis et al., 2018, Rotstein et al., 2018, Gene Disruption Project members, 2016-, Sarov et al., 2016, Gene Disruption Project members, 2015-, Ahmad et al., 2014, Chung and Andrew, 2014, Kim and Choe, 2014, Busser et al., 2012, Busser et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Zhu et al., 2012, Chatterjee et al., 2011, Pastor-Pareja and Xu, 2011, Kong et al., 2010, Strub et al., 2008, Tanentzapf et al., 2007, Philippakis et al., 2006)

ndg

(Artero et al., 2003)

Name Synonyms

Nidogen

(Santillán et al., 2025, Stricker et al., 2025, Kaltezioti et al., 2022, Meyer et al., 2022, Khadilkar et al., 2020, Töpfer and Holz, 2020, Zang et al., 2015, Kim and Choe, 2014, Drechsler et al., 2013, Pastor-Pareja and Xu, 2011, Urbano et al., 2011, Tanentzapf et al., 2007, Philippakis et al., 2006, Culi et al., 2004, Brown et al., 2000)

Nidogen-like

(Hynes and Zhao, 2000)

Nidogen/entactin

(Cammarato et al., 2011, Fakhouri et al., 2006, Kumagai et al., 1999)

nidogen

(Artero et al., 2003)

nidogen/entactin

(Chatterjee et al., 2011, Bunt et al., 2010)

Secondary FlyBase IDs

FBgn0033509
FBgn0046316

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36089

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z877

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A1Z877

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z877

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0026403

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36089/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0026403

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0026403

FlyMine - An integrated database for Drosophila genomics

FBgn0026403

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG12908

MARRVEL_MODEL - MARRVEL (model organism gene)

36089

Linkouts

BioGRID - A database of protein and genetic interactions.

61908

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0026403

DroID - A comprehensive database of gene and protein interactions.

FBgn0026403

DRSC - Results frm RNAi screens

FBgn0026403

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ndg_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0026403

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0026403

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12908

Flygut - An atlas of the Drosophila adult midgut

FBgn0026403

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0026403

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008043

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

36089

References (110)

The following external sites may use different assemblies or annotations than FlyBase.

-0.80

Report Sections