FlyBase Allele Report: Dmel\Ndg[1]

General Information

Symbol

Dmel\Ndg¹

Species

D. melanogaster

Name

FlyBase ID

FBal0344005

Feature type

allele

Associated gene

Dmel\Ndg

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Dai et al., 2018)

Mutagen

CRISPR/Cas9

(Dai et al., 2018)

Progenitor genotype

Cytology

Description

Deletion of two segments within Ndg ( 2R:10314236..10315587 and 2R:10316460..10319543 ), plus insertion of two nucleotides (TT) after position 2R:10316459 .

(Pastor J., 2019.2.13)

Deletion that removes part of the Ndg transcription unit, including the transcription start site. The CG34222 gene that is located in an intron of Ndg is not affected.

(Dai et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:10,314,236..10,315,587 [+]

Comment:

A 1352bp deletion that removes the translation start site of Ndg. One of two deletions in Ndg in mutant. CG34222 located in a downstream intron of Ndg is not affected.

(Pastor J., 2019.2.13)

delins

2R:10,316,460..10,319,543 [+]

Comment:

A 3084bp deletion that removes much of the coding region of Ndg. Two nucleotides (TT) are inserted at the site of the deletion. One of two deletions in Ndg in mutant. CG34222 located in a downstream intron of Ndg is not affected.

Inserted_sequence:

(Pastor J., 2019.2.13)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cold stress response | adult stage

(Dai et al., 2018)

abnormal cold stress response | adult stage (with Df(2R)BSC281)

(Dai et al., 2018)

abnormal size | larval stage

(Dai et al., 2018)

decreased fecundity | female

(Dai et al., 2018)

viable

(Dai et al., 2018)

Phenotype Manifest In

basement membrane | adult stage

(Dai et al., 2018)

basement membrane | larval stage

(Dai et al., 2018)

basement membrane | larval stage (with Df(2R)BSC281)

(Dai et al., 2018)

basement membrane | larval stage (with Ndg²)

(Dai et al., 2018)

basement membrane | larval stage (with Ndg^ΔRod-G3.1)

(Dai et al., 2018)

embryonic/larval fat body | larval stage

(Dai et al., 2018)

embryonic/larval fat body | larval stage (with Df(2R)BSC281)

(Dai et al., 2018)

embryonic/larval fat body | larval stage (with Ndg²)

(Dai et al., 2018)

embryonic/larval fat body | larval stage (with Ndg^ΔRod-G3.1)

(Dai et al., 2018)

indirect flight muscle cell | adult stage

(Dai et al., 2018)

lipid droplet | larval stage

(Dai et al., 2018)

Detailed Description

Statement

Reference

Ndg¹ mutants adults do not show any obvious morphological abnormalities; homozygous females lay significantly fewer egg but these do not show any obvious morphological defects. Ndg¹ homozygotes and Ndg¹/Df(2R)BSC281 transheterozygotes show a significantly longer chill coma recovery time, but no defects in flight or climbing activity under control conditions, as compared to controls.

Ndg¹ homozygotes exhibit basement membrane discontinuity in the larval fat body and in adult indirect flight muscles, but do not show any obvious basement membrane defects in the embryo, larval epidermis, imaginal discs, salivary glands, gut, muscles, VNC or follicular epithelium in the ovary; Ndg¹/Ndg², Ndg¹/Ndg^ΔRod-G3.1 and Ndg¹/Df(2R)BSC281 transheterozygotes also show discontinuity of the basement membrane in the larval fat body. The larval fat body of Ndg¹ homozygotes present significantly smaller lipid droplets, as compared to controls.

(Dai et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

LanA[+]/LanA²¹⁶, Ndg¹ has abnormal size | larval stage phenotype

(Dai et al., 2018)

LanA[+]/LanA¹⁶⁰, Ndg¹ has abnormal size | larval stage phenotype

(Dai et al., 2018)

Phenotype Manifest In

Other

Statement

Reference

LanA[+]/LanA²¹⁶, Ndg¹ has larval ventral nerve cord | larval stage phenotype

(Dai et al., 2018)

LanA[+]/LanA¹⁶⁰, Ndg¹ has larval ventral nerve cord | larval stage phenotype

(Dai et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Ndg¹ homozygotes also heterozygous for LanA¹⁶⁰ or LanA²¹⁶ show an elongated larval VNC, as compared to Ndg¹ homozygotes, LanA¹⁶⁰ heterozygotes or LanA²¹⁶ heterozygotes.

(Dai et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ndg¹ is rescued by Ndg^{ΔG1.UAS.EGFP}/Scer\GAL4^Cg.PA

(Dai et al., 2018)

Ndg¹ is rescued by Ndg^{ΔG2.UAS.EGFP}/Scer\GAL4^Cg.PA

(Dai et al., 2018)

Not rescued by

Ndg¹ is not rescued by Scer\GAL4^Cg.PA/Ndg^{ΔRod.UAS.EGFP}

(Dai et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ndg¹

(Matsubayashi et al., 2020, Pastor J., 2019.2.13, Dai et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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