Weak salto^GFP expression is observed at the tip of the sensory dendrites in chordotonal organs in embryos. Expression is strong in the testis and is observed at all stages of spermatogenesis, from spermatocytes to fully elongated spermatids. In spermatocytes, salto^GFP encages spermatocyte nuclei. It forms a halo around centrioles and is located at the primary like cilium above the centrioles. At the onset of meiosis, high levels are observed at the forming spindle poles and decorate the microtubules. After meiosis, salto^GFP decorates a half dome encircling the nuclei and anchored to the basal body and likely corresponds at the round spermatid stage to the fenestrated part of the nucleus surrounded by the microtubule network of the sperm manchette. After the beginning of flagellar elongation, salto^GFP is relocated both to the centriolar adjunct and to the acrosome. When spermatids are fully elongated and before the beginning of individualization, salto^GFP is mainly observed at the acrosome. In mature sperm, after individualization, this protein is not detected.