UASt regulatory sequences drive expression of ssx that is tagged at the N-terminal end with Tag:FLAG and three copies of Tag:HA.
lethal | heat sensitive, with Scer\GAL4da.G32
partially lethal - majority die, with Scer\GAL4da.G32
some die during embryonic stage, with Scer\GAL4da.G32
some die during larval stage, with Scer\GAL4da.G32
At 25[o]C, expression from two copies of ssxUAS.Tag:FLAG,Tag:HA under the control of Scer\GAL4da.G32 induces lethality. At 18[o]C and 20[o]C, expression from one copy induces partial lethality.