FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\His3.3ADendra2
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General Information
Symbol
Dmel\His3.3ADendra2
Species
D. melanogaster
Name
FlyBase ID
FBal0347211
Feature type
allele
Associated gene
Dmel\His3.3A
Associated Insertion(s)
TI{TI}His3.3ADendra2
Carried in Construct
Also Known As
H3.3A-Dendra2
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Shindo and Amodeo, 2019)
gene targeting by homologous recombination
(Shindo and Amodeo, 2019)
Progenitor genotype
Associated Insertion(s)
TI{TI}His3.3ADendra2
Cytology
Description

Sequence encoding a linker and the Dendra2 photoconvertible fluorescent protein has been inserted into the endogenous His3.3A locus, at the C-terminal end of the open reading frame.

(Shindo and Amodeo, 2019)
Allele components
Component
Use(s)
Tagged with
Dendra2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 3 -- 4
      organism

      Comment: increased with each nuclear cycle

      (Shindo and Amodeo, 2019)
      Additional Information
      Statement
      Reference

      The level of His3.3A reporter in the nucleus increases between nuclear cycles, doubling between nuclear cycle 10 and nuclear cycle 13 in early embryogenesis.

      (Shindo and Amodeo, 2019)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      His3.3ADendra2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)