FlyBase Allele Report: Dmel\brp[C-long.UAS.Tag:M(mCd8a),EGFP]

General Information

Symbol

Dmel\brp^{C-long.UAS.Tag:M(mCd8a),EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0347616

Feature type

allele

Associated gene

Dmel\brp

Associated Insertion(s)

Carried in Construct

P{UAS-mCD8-EGFP-brp.C-long}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Scholz et al., 2019)

Progenitor genotype

Carried in construct

P{UAS-mCD8-EGFP-brp.C-long}

Cytology

Description

UAS regulatory sequences drive expression of a fusion protein composed of Tag:M(mCd8a), EGFP and the 862 C-terminal amino acids of brp.

(Scholz et al., 2019)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

brp

Tagged with

EGFP

green fluorescent protein

Tag:M(mCd8a)

membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Phenotype Manifest In

axon | larval stage, with Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

embryonic/larval motor neuron, with Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

synaptic vesicle | larval stage, with Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Detailed Description

Statement

Reference

Expression of brp^{C-long.UAS.Tag:M(mCd8a),EGFP} under the control of Scer\GAL4^RapGAP1-OK6 in larvae results in significantly increased accumulation of synaptic vesicles in motorneuron axons, compared to controls.

(Scholz et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by Dysb^GD10754, Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by Scer\GAL4^RapGAP1-OK6/Syn^KK108941

(Scholz et al., 2019)

Suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/cpx^GD10482

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has abnormal neuroanatomy | larval stage phenotype, suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Dysb^GD10754, Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has abnormal neuroanatomy | larval stage phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/Syn^KK108941

(Scholz et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has synaptic vesicle | larval stage phenotype, non-enhanceable by Dysb^GD10754, Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has axon | larval stage phenotype, non-enhanceable by Dysb^GD10754, Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has embryonic/larval motor neuron phenotype, non-enhanceable by Dysb^GD10754, Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has synaptic vesicle | larval stage phenotype, non-enhanceable by Scer\GAL4^RapGAP1-OK6/Syn^KK108941

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has axon | larval stage phenotype, non-enhanceable by Scer\GAL4^RapGAP1-OK6/Syn^KK108941

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has embryonic/larval motor neuron phenotype, non-enhanceable by Scer\GAL4^RapGAP1-OK6/Syn^KK108941

(Scholz et al., 2019)

Suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has embryonic/larval motor neuron phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/cpx^GD10482

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has synaptic vesicle | larval stage phenotype, suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has axon | larval stage phenotype, suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has embryonic/larval motor neuron phenotype, suppressible by cpx^SH1/cpx¹²⁵⁷

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has synaptic vesicle | larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/cpx^GD10482

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has axon | larval stage phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/cpx^GD10482

(Scholz et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has synaptic vesicle | larval stage phenotype, non-suppressible by Dysb^GD10754, Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has axon | larval stage phenotype, non-suppressible by Dysb^GD10754, Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has embryonic/larval motor neuron phenotype, non-suppressible by Dysb^GD10754, Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has synaptic vesicle | larval stage phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/Syn^KK108941

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has axon | larval stage phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/Syn^KK108941

(Scholz et al., 2019)

Scer\GAL4^RapGAP1-OK6, brp^{C-long.UAS.Tag:M(mCd8a),EGFP} has embryonic/larval motor neuron phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/Syn^KK108941

(Scholz et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

brp^{C-long.UAS.Tag:M(mCd8a),EGFP} is rescued by brp^{C-tip.UAS.CFP,Tag:FLAG}/Scer\GAL4^RapGAP1-OK6

(Scholz et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

brp^{C-long.UAS.Tag:M(mCd8a),EGFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

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