FlyBase Allele Report: Dmel\RhoGAP100F[RA.UAS.3xTag:FLAG]

General Information

Symbol

Dmel\RhoGAP100F^{RA.UAS.3xTag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0348196

Feature type

allele

Associated gene

Dmel\RhoGAP100F

Associated Insertion(s)

Carried in Construct

P{UAS-RhoGAP100F.RA.3xFLAG}

Key Links

Transgenic product class

missense_variant

(Spinner et al., 2018)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Spinner et al., 2018)

Mutagen

in vitro construct

(Spinner et al., 2018)

Progenitor genotype

Carried in construct

P{UAS-RhoGAP100F.RA.3xFLAG}

Cytology

Description

UAS regulatory sequences drive expression of full-length RhoGAP100F that has been mutated to carry a R979A amino acid replacement (numbering according to isoform C, GenBank AAF57207.2). The protein is tagged with three copies of Tag:FLAG.

(Spinner et al., 2018)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

RhoGAP100F

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04

(Xu et al., 2024)

increased size | adult stage, with Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04

(Xu et al., 2024)

Phenotype Manifest In

adult antennal olfactory receptor neuron, with Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04

(Xu et al., 2024)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^Mz19 is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^Mz19, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

Suppressor of

Statement

Reference

RhoGAP100F^{RA.UAS.3xTag:FLAG}/Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04 is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

NOT Suppressor of

Statement

Reference

RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^Mz19 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^Mz19, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

Other

Statement

Reference

Hsap\RELA^AD.VT028327, RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^DBD.R22E04, Ten-m^UAS.Tag:V5 has abnormal neuroanatomy | adult stage phenotype

(Xu et al., 2024)

Hsap\RELA^AD.VT028327, RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^DBD.R22E04, Ten-m^UAS.Tag:V5 has increased size | adult stage phenotype

(Xu et al., 2024)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^Mz19 is a non-enhancer of adult antennal lobe phenotype of Scer\GAL4^Mz19, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^Mz19 is a non-enhancer of neuron projection | adult stage phenotype of Scer\GAL4^Mz19, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

Suppressor of

Statement

Reference

RhoGAP100F^{RA.UAS.3xTag:FLAG}/Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04 is a suppressor | partially of adult antennal lobe phenotype of Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

RhoGAP100F^{RA.UAS.3xTag:FLAG}/Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04 is a suppressor | partially of neuron projection | adult stage phenotype of Hsap\RELA^AD.VT028327, Scer\GAL4^DBD.R22E04, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

NOT Suppressor of

Statement

Reference

RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^Mz19 is a non-suppressor of adult antennal lobe phenotype of Scer\GAL4^Mz19, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^Mz19 is a non-suppressor of neuron projection | adult stage phenotype of Scer\GAL4^Mz19, Ten-m^UAS.Tag:V5

(Xu et al., 2024)

Other

Statement

Reference

Hsap\RELA^AD.VT028327, RhoGAP100F^{RA.UAS.3xTag:FLAG}, Scer\GAL4^DBD.R22E04, Ten-m^UAS.Tag:V5 has adult antennal olfactory receptor neuron phenotype

(Xu et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

RhoGAP100F^{RA.UAS.3xTag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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