FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Tak1K46R.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Tak1K46R.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0349275
Feature type
allele
Associated gene
Dmel\Tak1
Associated Insertion(s)
Carried in Construct
P{UAS-Tak1.K46R.FLAG}
Key Links
Transgenic product class
dominant_negative_variant
(Harris et al., 2018)
missense_variant
(Harris et al., 2018)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Harris et al., 2018)
missense_variant
(Harris et al., 2018)
Mutagen
in vitro construct
(Harris et al., 2018)
Progenitor genotype
Carried in construct
P{UAS-Tak1.K46R.FLAG}
Cytology
Description

UASt regulatory sequences drive expression of the full-length Tak1 open reading frame (derived from the LD42274 cDNA clone), that has been mutated to carry the K46R amino acid replacement (this is predicted to result in a dominant negative protein). The coding sequence is tagged at the C-terminal end with 3xTag:FLAG.

(Harris et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Tak1
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Third instar larvae expressing Tak1K46R.UAS.Tag:FLAG under the control of Scer\GAL4VGlut-OK371 display a significantly reduced mEPSP (mini excitatory postsynaptic potential) frequency compared to controls. Following high-frequency stimulation, motor neurons display profound short-term facilitation, in contrast to the synaptic depression characteristic of controls. There is no effect on the number of type I boutons and active zones per NMJ, or active zone density.

      Motor neurons of third instar larvae expressing Tak1K46R.UAS.Tag:FLAG under the control of either Scer\GAL4VGlut-OK371 or Scer\GAL4C57 display similar mEPSP (mini excitatory postsynaptic potential) and EPSP (excitatory postsynaptic potential) amplitudes and quantal content to controls.

      (Harris et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Tak1K46R.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)