FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\damUAS.NLS-MCPx2
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General Information
Symbol
Ecol\damUAS.NLS-MCPx2
Species
E. coli
Name
FlyBase ID
FBal0349501
Feature type
allele
Associated gene
Ecol\dam
Associated Insertion(s)
Carried in Construct
P{UAS-LT3-Dam-NLS-MCPx2}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Cheetham and Brand, 2018)
Progenitor genotype
Carried in construct
P{UAS-LT3-Dam-NLS-MCPx2}
Cytology
Description

UASt regulatory sequences drive expression of two open reading frames separated by stop codons (the upstream ORF is simply present to reduce translation of the Tag:Dam-containing downstream ORF). The downstream ORF comprises Tag:Dam, a nuclear localization signal, a Tag:MYC epitope and a tandem dimer of Tag:MCP and is one component of the 'RNA-DamID' system; when expressed together with a lncRNA tagged with Tag:MS2 (binding target of Tag:MCP), it is recruited to sites of lncRNA-chromatin interaction, resulting in methylation of the DNA by Tag:Dam in the vicinity of the interaction site.

(Cheetham and Brand, 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Tag:Dam
Tagged with
Tag:MCP
Tag:MYC
Tag:NLS(Unk)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ecol\damUAS.NLS-MCPx2
      Name Synonyms
      Secondary FlyBase IDs
        References (1)