FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-LT3-Dam-NLS-MCPx2}
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General Information
Symbol
P{UAS-LT3-Dam-NLS-MCPx2}
FlyBase ID
FBtp0135283
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\damUAS.NLS-MCPx2
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\damUAS.NLS-MCPx2
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Tag:Dam
Tagged with
Tag:MCP
Tag:MYC
Tag:NLS(Unk)
Description

UASt regulatory sequences drive expression of two open reading frames separated by stop codons (the upstream ORF is simply present to reduce translation of the Tag:Dam-containing downstream ORF). The downstream ORF comprises Tag:Dam, a nuclear localization signal, a Tag:MYC epitope and a tandem dimer of Tag:MCP and is one component of the 'RNA-DamID' system; when expressed together with a lncRNA tagged with Tag:MS2 (binding target of Tag:MCP), it is recruited to sites of lncRNA-chromatin interaction, resulting in methylation of the DNA by Tag:Dam in the vicinity of the interaction site.

(Cheetham and Brand, 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-LT3-Dam-NLS-MCPx2}
(FlyBase, 1992-)
UAS-Dam-NLS-MCPx2
(Cheetham and Brand, 2018)
Secondary FlyBase IDs
    References (3)