A Tag:HA tag has been inserted into the first coding exon (insertion is between codons 87 and 88) of the endogenous locus of the bnlsfGFP.endo mutant, resulting in a doubly-tagged locus. In addition, putative cleavage site PSC1 with the bnl coding sequence has been mutated.
The wing discs of bnlHA-1.sfGFP-3.M1.endo homozygotes exhibit stunted air sac primordium growth.