Fili^CRISPR homozygotes have mis-targeting of some VM5 projection neuron (PN) dendrites to a more dorsal and posterior glomerulus of the antennal lobe, compared to controls; some Va1v olfactory receptor neuron (ORN) axons are mis-targeted to the Va1d glomerulus and some DC1 ORN axons are mis-targeted to a site lateral to the DC1 glomerulus, unlike controls; the VA7l ORN target region in the antennal lobe is mis-shapen; there is no mis-targeting of VA6, DA2, DC2, VA2, VM6, VM2, DL3 or VC3 PNs, or of DA4m, DC2, DM3, VA5, DM4, DM6, VC4, DA1, VA1d or VM5v ORNs, compared to controls.

Fili^CRISPR homozygous MARCM neuroblast clones (induced using hs-FLP in larvae at 1 d post-hatching) produce VM5 PNs with correctly-targeted dendrites, compared to controls; when affected (Fili^CRISPR homozygous) neurons are limited to the anterodorsal neuroblast lineage (including VA1d and DC3 PNs) some Va1v ORN axons are mis-targeted to the Va1d glomerulus, unlike controls, with no mis-targeting to the DC3 glomerulus; there is no mistargeting of Va1v ORN axons when clones are limited to the lateral neuroblast lineage (including DA1 PNs).

The presence of Fili^CRISPR homozygous MARCM ORN clones (induced using ey-FLP in combination with a cell lethal strategy) results in mis-targeting of some VM5 PN dendrites to a more dorsal and posterior glomerulus of the antennal lobe, compared to controls; there is no effect on targeting of VM5d, VM5v, or Va1v ORN axons, compared to controls.