FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\cyst1
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General Information
Symbol
Dmel\cyst1
Species
D. melanogaster
Name
FlyBase ID
FBal0356946
Feature type
allele
Associated gene
Dmel\cyst
Associated Insertion(s)
TI{TI}cyst1
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Silver et al., 2019)
gene targeting by homologous recombination
(Silver et al., 2019)
Progenitor genotype
Associated Insertion(s)
TI{TI}cyst1
Cytology
Description

Deletion (coordinates 2L:19503155..19506410 ) that removes most of the cyst coding sequence (deletion includes the DH and PH domains and entire C-terminal part of the protein). A Avic\GFP3xP3.cUa marker has been inserted in place of the deleted sequence.

(Silver et al., 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2L:19,503,155..19,506,410 [-]
Comment:

A 3256bp deletion (reported coordinates 2L:19503155..19506410 ) that removes most of the cyst coding sequence. A Avic\GFP3xP3.cUa marker has been inserted in place of the deleted sequence.

(Silver et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Zygotic cyst1 mutants are not embryonic lethal but die at later stages of development.

      About half of embryos derived from cyst1 germline clones and crossed to cyst1/+ males show two phenotypes. About one half shows prominent epidermal defects, namely large number of epithelial vesicles and shields of cuticle of various sizes. The other half is not embryonic lethal.

      (Silver et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Double cyst1, l(2)gl4 maternal-zygotic mutants show embryonic cuticle defects that are similar to those observed in l(2)gl4 single maternal-zygotic mutants (i.e. head or head cuticle morphogenesis), and milder than those observed in cyst1 single maternal-zygotic mutants.

      (Silver et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      cyst1
      (Silver et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)