Genomic fragment that encompasses the cyst gene and includes its promoter (derived from the BACR27M12 BACPAC clone). Sequence encoding the C-terminal portion of the coding sequence has been deleted. A S(GGGGS)[[2]] linker followed by a sfGFP tag has been inserted at the C-terminal end of the cyst coding sequence.
cystR.ΔC.C-sfGFP fails to rescue cystHMS01750
Maternal expression of cystHMS01750 under the control of Scer\GAL4VP16.mat.αTub67C leads to embryonic lethality and cuticle defects, both of which cannot be rescued by cystR.ΔC.C-sfGFP.