Insertion of TI{CRISPaint.T2A-GAL4} sequence into the 5' part of the Fkbp12 coding sequence. The insertion is in antisense orientation (T2A-GAL4 sequence within the inserted sequence cassette is in the opposite orientation to transcription of Fkbp12); as expected, no GAL4 expression is seen. A 30bp deletion is associated with the site of the TI{CRISPaint.T2A-GAL4} insertion.
Insertion of TI{CRISPaint.T2A-GAL4} sequence into the 5' part of the Fkbp12 coding sequence. The insertion is in antisense orientation (T2A-Scer\GAL4 sequence within the inserted sequence cassette is in the opposite orientation to transcription of Fkbp12). A 30bp deletion is associated with the site of the TI{CRISPaint.T2A-GAL4} insertion.