FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\shgS4A.GFP
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General Information
Symbol
Dmel\shgS4A.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0358646
Feature type
allele
Associated gene
Dmel\shg
Associated Insertion(s)
TI{TI}shgS4A.GFP
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
recombination
(Chen et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}shgS4A.GFP
Cytology
Description

shg in which all four serine residues are mutated to alanine (non-phosphorylatable) and tagged at the C-terminal end with GFP has been knocked in to a founder shg knockout line containing an attP site.

(Chen et al., 2017)
Allele components
Component
Use(s)
Tagged with
GFP
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      shgS4A.GFP homozygous embryos exhibit cuticle defects.

      As a result of whole-cell FRAP assay, maternal and zygotic shgS4A.GFP homozygous mutant embryos (derived from mothers containing germline shgS4A.GFP clones) exhibit significantly increased DE-Cad biosynthetic turnover in polarized epithelial cells at stage 15 embryos.

      Maternal and zygotic shgS4A.GFP homozygous mutant embryos (derived from mothers containing germline shgS4A.GFP clones) develop normally until germband retraction after which the epidermis appears to break down during dorsal closure.

      (Chen et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DE-CadS4A::GFP
      (Chen et al., 2017)
      shgS4A.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)