shg in which 22aa within the conserved serine cluster is deleted and tagged at the C-terminal end with GFP has been knocked in to a founder shg knockout line containing an attP site.
abnormal size | somatic clone | third instar larval stage
lethal (with shg2)
lethal - all die during embryonic stage
adherens junction | somatic clone | third instar larval stage
embryonic/first instar larval cuticle | embryonic stage
wing disc | somatic clone | third instar larval stage
Third instar larval wing disc clones of shgΔS.GFP are small, rounded and fail to form discernible adherens junctions.
shgΔS.GFP homozygous embryos exhibit severe loss-of-cuticle phenotype.