Expression is visible in 3-4 clusters of cells at the outer optic anlagen area at mid-second instar. This expression is gone by third instar. Expression is also seen in small clusters in the antennal lobe. Moderate expression observed in the antennal lobe and mushroom body in third instar. Expression in these structures is first observed in the second instar. Staining is also seen in a few cells in the subesophageal ganglion.