FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Tbc1d8-92xsgRNA.U6
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General Information
Symbol
Dmel\Tbc1d8-92xsgRNA.U6
Species
D. melanogaster
Name
FlyBase ID
FBal0358804
Feature type
allele
Associated gene
Dmel\Tbc1d8-9
Associated Insertion(s)
Carried in Construct
M{U6-Tbc1d8-9.2xsgRNA}
Key Links
Transgenic product class
sgRNA
(Kampf et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
sgRNA
(Kampf et al., 2019)
Mutagen
in vitro construct
(Kampf et al., 2019)
Progenitor genotype
Carried in construct
M{U6-Tbc1d8-9.2xsgRNA}
Cytology
Description

Ubiquitously expresses two sgRNAs, each of which targets Tbc1d8-9.

(Kampf et al., 2019)
Allele components
Component
Use(s)
Encoded product / tool
Tbc1d8-9
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  nephrotic syndrome type 20
      CEA
      (Kampf et al., 2019)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Nephrocyte-specific CRISPR/Cas9-mediated mutagenesis of Tbc1d8-9 (by expressing Spyo\Cas9UAS.P2 under the control of Scer\GAL4Ugt36A1.PK, together with Tbc1d8-92xsgRNA.U6) leads to impaired nephrocyte function (decreased uptake of FITC-albumin), associated with slit diagram localization defects with the formation of additional, ectopic slit diaphragms deeper within the membrane invaginations.

      (Kampf et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Tbc1d8-92xsgRNA.U6
      Name Synonyms
      Secondary FlyBase IDs
        References (1)