FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Rnor\Camk2aCamuiα.UAS
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General Information
Symbol
Rnor\Camk2aCamuiα.UAS
Species
R. norvegicus
Name
FlyBase ID
FBal0359116
Feature type
allele
Associated gene
Rnor\Camk2a
Associated Insertion(s)
Carried in Construct
P{UAS-Camuiα}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Shakiryanova et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Camuiα}
Cytology
Description

UASt regulatory sequences drive expression of 'Camuiα' a fluorescence resonance energy transfer (FRET)-based reporter of CamKII kinase activity. 'Camuiα' (GenBank:AY928551) consists of the entire Rnor\Camk2a coding sequence tagged at the N-terminal end with Venus and at the C-terminal end with a cyan fluorescent protein that has enhanced brightness (carries the S175G mutation). Upon activation, the Rnor\Camk2a protein in Camuiα undergoes a conformational change that reduces FRET between the two fluorophores; this FRET change reflects calmodulin binding and autophosphorylation at residue T286 (details from PMID:15788767).

(Shakiryanova et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rnor\Camk2a
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Rnor\Camk2aCamuiα.UAS
      Zzzz\CamuialphaScer\UAS.cSa
      Zzzz\CamuialphaUAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Shakiryanova
      Secondary FlyBase IDs
      • FBal0359073
      • FBal0284706
      References (1)