FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{UAS-Camuiα}
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General Information
Symbol
P{UAS-Camuiα}
FlyBase ID
FBtp0084882
Feature type
transgenic_transposable_element
Component Allele(s)
Rnor\Camk2aCamuiα.UAS
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Rnor\Camk2aCamuiα.UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rnor\Camk2a
Description

UASt regulatory sequences drive expression of 'Camuiα' a fluorescence resonance energy transfer (FRET)-based reporter of CamKII kinase activity. 'Camuiα' (GenBank:AY928551) consists of the entire Rnor\Camk2a coding sequence tagged at the N-terminal end with Venus and at the C-terminal end with a cyan fluorescent protein that has enhanced brightness (carries the S175G mutation). Upon activation, the Rnor\Camk2a protein in Camuiα undergoes a conformational change that reduces FRET between the two fluorophores; this FRET change reflects calmodulin binding and autophosphorylation at residue T286 (details from PMID:15788767).

(Shakiryanova et al., 2011)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Camui
(Shakiryanova et al., 2011)
P{UAS-Camui.S}
(FlyBase, 1992-)
P{UAS-Camuiα}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (4)