FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\RELAwg-T2A-p65
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General Information
Symbol
Hsap\RELAwg-T2A-p65
Species
H. sapiens
Name
FlyBase ID
FBal0362687
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
TI{2A-p65(AD)::Zip+}wgT2A-p65
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Ewen-Campen et al., 2020)
gene targeting by homologous recombination
(Ewen-Campen et al., 2020)
Progenitor genotype
Associated Insertion(s)
TI{2A-p65(AD)::Zip+}wgT2A-p65
Cytology
Description

A sequence cassette that encodes the T2A peptide plus the p65(AD)::Zip+ split driver component has been inserted into the endogenous wg locus, in frame within the first exon. A loxP cassette encoding a Disc\RFPDsRed(T1).3xP3.cHa marker has also been inserted during the mutagenesis event, downstream of the split driver sequence. The presence of the T2A sequence is expected to truncate the endogenous wg protein and to allow the separate expression of p65(AD)::Zip+ under the control of the endogenous wg regulatory sequences.

(Ewen-Campen et al., 2020)
Allele components
Component
Use(s)
Inserted element
TI{2A-p65(AD)::Zip+}
Encoded product / tool
p65(AD)::Zip+
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hsap\RELAwg-T2A-p65
      Name Synonyms
      Secondary FlyBase IDs
        References (1)