FlyBase Allele Report: Dmel\wg[gRNAx2]

General Information

Symbol

Dmel\wg^gRNAx2

Species

D. melanogaster

Name

FlyBase ID

FBal0363989

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

PBac{foxo-dCas9::VPR,wg.gRNAx2}

PBac{wg(ΔC1)-dCas9::VPR,wg.gRNAx2}

Key Links

Transgenic product class

sgRNA

(Maselko et al., 2020)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Maselko et al., 2020)

Mutagen

in vitro construct

(Maselko et al., 2020)

Progenitor genotype

Carried in construct

PBac{foxo-dCas9::VPR,wg.gRNAx2}

PBac{wg(ΔC1)-dCas9::VPR,wg.gRNAx2}

Cytology

Description

Expresses two sgRNAs (the same as in P{TOE.GS00125}), both of which target the wg promoter.

(Maselko et al., 2020)

Allele components

Component

Use(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage, with Spyo\Cas9^{dCas9::VPR.wg-ΔC1}

(Maselko et al., 2020)

lethal - all die before end of larval stage, with Spyo\Cas9^{dCas9::VPR.αTub84B}

(Maselko et al., 2020)

lethal - all die before end of P-stage, with Spyo\Cas9^{dCas9::VPR.foxo}

(Maselko et al., 2020)

lethal - all die before end of P-stage, with Spyo\Cas9^{dxCas9-3.7::VPR.αTub84B}

(Maselko et al., 2020)

viable, with Spyo\Cas9^{dCas9::VPR.wg-ΔC1}

(Maselko et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

wg^gRNAx2

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close