FlyBase Allele Report: Dmel\bol[intron3.KO]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\bol^intron3.KO

General Information

Symbol

Dmel\bol^intron3.KO

Species

D. melanogaster

Name

FlyBase ID

FBal0365224

Feature type

allele

Associated gene

Associated Insertion(s)

TI{TI}bol^intron3.KO

Carried in Construct

Key Links

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Gao et al., 2020)

Progenitor genotype

TI{TI}bol^KO.attP

(Gao et al., 2020)

Associated Insertion(s)

TI{TI}bol^intron3.KO

Cytology

Description

DNA has been re-integrated into the attP site in the TI{TI}bol^KO.attP insertion, reconstituting a bol locus that is missing intron 3. An attR site is present upstream of exon 2 and a loxP site is present downstream of exon 8'. The two major circRNAs that are normally produced from the bol locus (circEx2-3 and circEx4-7) are absent in this mutant, while the levels of linear bol mRNA and bol protein are similar to wild type.

(Gao et al., 2020)

Allele components

Component

Use(s)

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity | male

(Gao et al., 2020)

increased cell death | spermatogenesis

(Gao et al., 2020)

male semi-sterile | progressive | heat sensitive

(Gao et al., 2020)

(Gao et al., 2020)

Phenotype Manifest In

nucleus | spermatogenesis

(Gao et al., 2020)

(Gao et al., 2020)

spermatozoon | decreased number

(Gao et al., 2020)

Detailed Description

Statement

Reference

bol^intron3.KO mature sperm (in the seminal vesicle) show abnormal nuclear morphology and are frequently apoptotic.

(Gao et al., 2020)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

bol^intron3.KO has male semi-sterile | progressive phenotype, suppressible | partially by Hsp60C^KO/Hsp60C[+]

(Gao et al., 2020)

bol^intron3.KO has male semi-sterile | progressive phenotype, suppressible | partially by Hsc70-4^KO/Hsc70-4[+]

(Gao et al., 2020)

bol^intron3.KO has male semi-sterile | progressive phenotype, suppressible | partially by Mmus\Boll^{βTub85D.circEx3-6}

(Gao et al., 2020)

bol^intron3.KO has male semi-sterile | progressive phenotype, suppressible | partially by Mmus\Boll^{βTub85D.circEx3-6.m2mut}

(Gao et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

bol^intron3.KO is partially rescued by bol^{βTub85D.circEx2-3}

(Gao et al., 2020)

bol^intron3.KO is partially rescued by bol^{βTub85D.circEx2-3}/bol^{βTub85D.circEx4-7}

(Gao et al., 2020)

bol^intron3.KO is partially rescued by bol^{βTub85D.circEx4-7}

(Gao et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

bol^intron3.KO

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections