FlyBase Allele Report: Dmel\Kpc2[Z2-5905]

General Information

Symbol

Dmel\Kpc2^Z2-5905

Species

D. melanogaster

Name

FlyBase ID

FBal0367429

Feature type

allele

Associated gene

Dmel\Kpc2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Li et al., 2020)

Progenitor genotype

Cytology

Description

A splice site mutation in Kpc2 affecting the splice donor site of a small coding exon contained in transcript C (2491 base pairs, 561 amino acids) and the testes-specific transcript B (1778 base pairs, 435 amino acids). The mutation results in the inclusion of the intron between exons 1 and 2, which is predicted to result in a novel protein and a premature stop codon after 61 amino acids. Transcript C is not detected in Kpc2^Z2-5905 flies, presumably due to nonsense mediated decay.

(Li et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:19,671,296..19,671,296 [+]

Comment:

Nucleotide substitution: GT changed to AT in the splice donor. The mutation affects the first coding exon of Kpc2-RB and the small (second) coding exon of Kpc2-RB.

(Li et al., 2020)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male sterile

(Li et al., 2020)

Phenotype Manifest In

spermatozoon

(Li et al., 2020)

Detailed Description

Statement

Reference

After mating Kpc2^Z2-5905 sperm does not reach the seminal receptacle and are restricted to the uterus.

(Li et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Kpc2^Z2-5905 is rescued by Kpc2^+t6.3

(Li et al., 2020)

Partially rescued by

Kpc2^Z2-5905 is partially rescued by Kpc2^Tag:V5

(Li et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Kpc2^Z2-5905

Z2-5905

(Li et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close