FB2026_02 , released June 18, 2026
Allele: Dmel\RetMEN2B.UAS.Tag:LOV(VfAU1)
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General Information
Symbol
Dmel\RetMEN2B.UAS.Tag:LOV(VfAU1)
Species
D. melanogaster
Name
FlyBase ID
FBal0368242
Feature type
allele
Associated gene
Dmel\Ret
Associated Insertion(s)
Carried in Construct
P{UAS-Ret.MEN2B.AU1LOV}
Key Links
Genomic Maps

Transgenic product class
missense_variant
(Ingles-Prieto et al., 2021)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Ingles-Prieto et al., 2021)
Mutagen
in vitro construct
(Ingles-Prieto et al., 2021)
Progenitor genotype
Carried in construct
P{UAS-Ret.MEN2B.AU1LOV}
Cytology
Description

UASt regulatory sequences drive expression of full-length Ret that carries a M955T amino acid substitution in the kinase domain; this change is orthologous to a human RET variant that has been identified in patients with multiple endocrine neoplasia type 2B. In addition, the coding sequence is tagged at the C-terminal end with Tag:LOV(VfAU1) (a light-activated dimerization switch); this allows the active mutant Ret receptor (a dimer) to be induced in a light-dependent manner.

(Ingles-Prieto et al., 2021)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ret
Tagged with
Tag:LOV(VfAU1)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:21,184,017..21,184,017 [-]
Nucleotide change:

T21184017C

Amino acid change:

M955T | Ret-PA; M955T | Ret-PB; M955T | Ret-PC

Reported amino acid change:

M955T

Comment:

Analogous M918T mutation in human RET implicated in multiple endocrine neoplasia and thyroid carcinoma; mutation carried on in vitro construct.

(Ingles-Prieto et al., 2021)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Ingles-Prieto et al., 2021)
      RET:p.Met918Thr
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      RET:p.Met664Thr
      Associated human disease model(s)
      External database links
      ClinVar: RET_13919
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      RetMEN2B.UAS.Tag:LOV(VfAU1)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)