hypomorphic allele - molecular evidence

The NimC1^del allele comprises 2 independent deletions: a 6 bp microdeletion from nucleotides 2264 to 2270 ( 2L:13974483-2L:13974489 ), and a 355 bp deletion from nucleotides 1582 to 1937 ( 2L:13974817-2L:13975172 ) accompanied by a 5 bp insertion (at position 2L:13974817 ). The 355 bp deletion and the 5 bp insertion generate a frameshift mutation in both NimC1-RA and NimC1-RB transcripts. As a result of the frameshift, the insertion is followed by a newly arisen sequence of 12 codons and a UGA stop codon, predicting truncated products of only 307 (NimC1-PA) and 309 (NimC1-PB) amino acids, compared to the normal 620 and 622 amino acids. The truncated proteins lack their C-terminal regions, including their intracellular and transmembrane domains, as well as four NIM repeats from the extracellular region.