FB2026_02 , released June 18, 2026
Allele: Dmel\twi32xSunTag.128xTag:MS2
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General Information
Symbol
Dmel\twi32xSunTag.128xTag:MS2
Species
D. melanogaster
Name
FlyBase ID
FBal0368350
Feature type
allele
Associated gene
Dmel\twi
Associated Insertion(s)
Carried in Construct
PBac{twi.32xSunTag.128xMS2}
Key Links
Transgenic product class
genomic_DNA
(Dufourt et al., 2021)
wild_type
(Dufourt et al., 2021)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Dufourt et al., 2021)
wild_type
(Dufourt et al., 2021)
Mutagen
in vitro construct
(Dufourt et al., 2021)
Progenitor genotype
Carried in construct
PBac{twi.32xSunTag.128xMS2}
Cytology
Description

twi genomic fragment that includes the proximal enhancer, promoter and transcription unit (including 5'UTR and 3'UTR). 32 repeats of the SunTag(v4) peptide have been inserted at the N-terminal end of the coding sequence (this allows visualization of the protein). 128 copies of the Tag:MS2 stem loop have been inserted at the N-terminal end of the 3'UTR (this allows visualization of the transcript).

(Dufourt et al., 2021)
Allele components
Component
Use(s)
Regulatory region(s)
twi
Encoded product / tool
twi
Tagged with
SunTag(v4)
Tag:MS2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      twi32xSunTag.128xTag:MS2
      Name Synonyms
      Secondary FlyBase IDs
        References (1)