FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Experimental tool: SunTag(v4)
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General Information
Symbol
SunTag(v4)
FlyBase ID
FBto0000738
Name
Description
Description

SunTag(v4) is a 19 residue peptide (EELLSKNYHLENEVARLKK) that is bound robustly and specifically by the scFv(GCN4) single-chain variable fragment antibody (PMID:25307933). SunTag(v4) is an optimized version of a scFv(GCN4) compatible epitope originally derived (PMID:10644744) from the Saccharomyces cerevisiae GCN4 gene ( SGDID:S000000735 ). If a protein of interest is tagged with a repeating array of the SunTag(v4) epitope, multiple copies of scFv(GCN4) can be recruited to the SunTag(v4) array. This property of 'signal amplification via protein multimerization' has a number of applications in vivo. For example, if the scFv(GCN4) antibody sequence is fused to a fluorescent protein, expression of a protein of interest tagged with a SunTag(v4) array can be visualized with very high sensitivity, even at the single molecule level (PMID:25307933).

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Other related tools (1)
Transgenic Constructs
Insertions ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
SunTag(v4)
Name Synonyms
Secondary FlyBase IDs
    References (6)