A knockout mutation in which the first exon of aus containing the initiating methionine is replaced with a sequence cassette that encodes 1. a single attP site, and 2. a loxP cassette containing the Disc\RFPDsRed(T1).3xP3.cHa marker.
The first exon of aus containing the initiating methionine is replaced with a selectable marker, DsRed(Unk).