FlyBase Allele Report: Dmel\RpS5b[CRISPR]

General Information

Symbol

Dmel\RpS5b^CRISPR

Species

D. melanogaster

Name

FlyBase ID

FBal0370558

Feature type

allele

Associated gene

Dmel\RpS5b

Associated Insertion(s)

TI{TI}RpS5b^CRISPR

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Jang et al., 2021)

Mutagen

CRISPR/Cas9

(Jang et al., 2021)

gene targeting by homologous recombination

(Jang et al., 2021)

Progenitor genotype

Associated Insertion(s)

TI{TI}RpS5b^CRISPR

Cytology

Description

The open reading frame of the endogenous RpS5b locus has been deleted and replaced by a cassette that encodes 1. a Disc\RFP^{DsRed(T1).3xP3.cHa} marker flanked by loxP sites (allowing subsequent removal of this marker from a targeted locus), and 2. a single attP site.

(Jang et al., 2021)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis

(Jang et al., 2021)

decreased fecundity | female

(Jang et al., 2021)

female sterile

(Jang et al., 2021)

viable

(Jang et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

In RpS5b^CRISPR homozygous females, egg chambers do not complete stage 8; stage 7/8 egg chambers exhibit an abnormal nuclear structure and multiple layered follicle cells within the posterior region; stage 8 oocytes do not accumulate yolk granules; stage 7 RpS5b^CRISPR egg chambers display increased frequency of follicle cells undergoing mitosis, which is only observed in the posterior region of the egg chamber; stage 8 oocytes show a reduced and unpolarized microtubule cytoskeleton.

(Jang et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RpS5b^CRISPR has decreased fecundity | female phenotype, suppressible by RpS5a^UAS.cJa/Scer\GAL4^{VP16.mat.αTub67C}

(Jang et al., 2021)

RpS5b^CRISPR has female sterile phenotype, suppressible by RpS5a^UAS.cJa/Scer\GAL4^{VP16.mat.αTub67C}